Combinatorial Modification of Degradable Polymers Enables Transfection of Human Cells Comparable to Adenovirus
Advanced Materials2007Vol. 19(19), pp. 2836–2842
Citations Over TimeTop 10% of 2007 papers
Jordan J. Green, Gregory T. Zugates, Nathan C. Tedford, Yu Huang, Linda G. Griffith, Douglas A. Lauffenburger, Janet A. Sawicki, Róbert Langer, Daniel G. Anderson
Abstract
End-modified poly(β-amino ester)s, easy-to-synthesize degradable polymers, are able to deliver DNA to primary human cells at levels comparable to adenovirus and two orders of magnitude better than the commonly used non-viral vector, polyethylenimine. Small structural changes are found to affect multiple steps of gene delivery including the DNA binding affinity, nanoparticle size, intracellular DNA uptake, and final protein expression. In vivo, these polymer modifications enhance DNA delivery to ovarian tumors.
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