Genetically Programmed In Vivo Packaging of Protein Cargo and Its Controlled Release from Bacteriophage P22
Angewandte Chemie International Edition2011Vol. 50(32), pp. 7425–7428
Citations Over TimeTop 1% of 2011 papers
Abstract
Packed and ready to go: A scaffold protein (SP) aids the assembly of Salmonella typhimuriam bacteriophage P22 into a capsid, with encapsulation of the SP. This natural process was exploited by using an engineered molecular system to fuse a fluorescent protein cargo (green in the picture) to a portion of the SP (yellow), which templated accurate spontaneous assembly. Heating of the capsids and treatment with thrombin released the SP but not the cargo.
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