Molar mass distribution by size exclusion chromatography: Comparison of multi‐angle light scattering and universal calibration
Citations Over Time
Abstract
ABSTRACT Series of polymers of various molar mass, chemical composition, and molecular architecture was analyzed by size exclusion chromatography (SEC) coupled with a multi‐angle light scattering (MALS) photometer and an online viscometer. The molar mass averages were determined from the signal of MALS or calculated from the intrinsic viscosity and universal calibration. The comparison of the obtained results showed significant differences between the two methods. The MALS detection was shown to be more accurate for the determination of the weight‐average molar mass and less vulnerable to the spreading of polymer peak by band broadening. The universal calibration can yield more accurate estimation of the number‐average molar mass of branched polymers. It is also significantly more accurate for the characterization of fluorescent polymers than MALS with a regular laser of 660 nm. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019 , 136 , 47561.
Related Papers
- → Solution properties of hyaluronic acid and comparison of SEC‐MALS‐VIS data with off‐line capillary viscometry(2010)52 cited
- → The Influence of Temperature on the Characterization of Water-Soluble Polymers Using Asymmetric Flow Field-Flow-Fractionation Coupled to Multiangle Laser Light Scattering(2000)33 cited
- → Co-elution effects can influence molar mass determination of large macromolecules with asymmetric flow field-flow fractionation coupled to multiangle light scattering(2017)18 cited
- → Polysaccharide Characterization by Flow Field-Flow Fractionation-Multiangle Light Scattering: Initial Studies of Modified Starches(2002)23 cited
- Asymmetrical Flow Field-Flow Fractionation and Multiangle Light Scattering for Molar Mass Analysis of Polysaccharide Derivatives(2003)