Pepsin immobilization by electrospinning of poly(vinyl alcohol) nanofibers
Citations Over Time
Abstract
Abstract The enzyme pepsin (E.C. 3.4.23.1, strength ≥400 μ/mg) was immobilized in poly(vinyl alcohol) (PVA) nanofibers for the first time by the electrospinning technique. The morphology of the nanofibers analyzed by SEM showed a diameter in the nanometric range of 70–165 nm. The presence of pepsin in the nanofibrous matrix was proved by infrared spectroscopy (FTIR). The immobilization process was carried out through crosslinking by exposure to glutaraldehyde (GA) vapor. The thermal analysis by differential scanning calorimetry (DSC) showed the effect of crosslinking time and enzyme concentration on the glass transition temperature ( T g ) and heat of fusion ( H f ) of the nanofibrous matrix. The optimum catalytic performance of the immobilized pepsin was reached at pH 3, 1 h of crosslinking and 25% of enzyme concentration. The enzymatic activity of immobilized pepsin reached 96% of the crude enzyme. The immobilization process allowed 4 cycles of reuse and up to 10 days of storage for the crosslinked samples. PVA nanofibers was successfully used as vehicle for the encapsulation and immobilization of the pepsin enzyme.
Related Papers
- → Acid catalysed cross-linking of poly vinyl alcohol (PVA) by glutaraldehyde: effect of crosslink density on the characteristics of PVA membranes used in single chambered microbial fuel cells(2015)224 cited
- → Adsorption of tetracycline from water using glutaraldehyde-crosslinked electrospun nanofibers of chitosan/poly(vinyl alcohol)(2018)50 cited
- → Poly(vinyl alcohol) hydrogels: Their synthesis and steps towards control of electroendosmosis(2003)12 cited
- → Synthesis of spherical porous cross-linked glutaraldehyde/poly(vinyl alcohol) hydrogels(2016)4 cited
- STUDY ON THE CONDITION OF THE IMMOBILIZING OF PEPSIN(2004)