A new, simple, highly scalable, and efficient protocol for genomic DNA extraction from diverse plant taxa
Applications in Plant Sciences2021Vol. 9(3), pp. e11413–e11413
Citations Over TimeTop 12% of 2021 papers
Evgeny V. Mavrodiev, Christopher Dervinis, W. Mark Whitten, Matthew A. Gitzendanner, Matias Kirst, Sangtae Kim, Taliesin J. Kinser, Pamela S. Soltis, Douglas E. Soltis
Abstract
Application of the protocol to various plant species yielded DNA of high quality in less than two hours and can be adjusted to extract DNA at large (maxi-preps) or small (96-well minipreps) scales. We anticipate that our method will be of wide utility for rapidly isolating large quantities of quality genomic DNA from diverse plant species and will have broad applications in phylogenetic studies utilizing PCR and short-read DNA sequencing.
Related Papers
- Extraction and random primer PCR detection of genomic DNA of parasitic mites Demodex folliculorum and Demodex brevis(Acari:Demodicidae)(2009)
- → Comparison of DNA Isolation Kits to Extract DNA from Whole Blood Samples(2006)1 cited
- Improvement of bacterial genomic DNA extraction in clinical specimens(2007)
- COMPARATIVE STUDY OF EXTRACTION METHOD FOR GENOMIC DNA IN MAT RUSH(2008)
- Optimization of Extraction Methods for Genomic DNA in Eomecon chinantha Hance.(2010)