Intracellular effect of ultrashort electrical pulses
Citations Over TimeTop 1% of 2001 papers
Abstract
A simple electrical model for biological cells predicts an increasing probability for electric field interactions with cell substructures of prokaryotic and eukaryotic cells when the electric pulse duration is reduced into the sub-microsecond range. The validity of this hypothesis was verified experimentally by applying electrical pulses with electric field intensities of up to 5.3 MV/m to human eosinophils in vitro. When 3-5 pulses of 60 ns duration were applied to human eosinophils, intracellular granules were modified without permanent disruption of the plasma membrane. In spite of the extreme electrical power levels applied to the cells thermal effects could be neglected because of the ultrashort pulse duration. The intracellular effect extends conventional electroporation to cellular substructures and opens the potential for new applications in apoptosis induction, gene delivery to the nucleus, or altered cell functions, depending on the electrical pulse conditions.
Related Papers
- → A numerical study of the effect of pulse duration on preventing particle generation during the AlN pulsed MOCVD process(2022)4 cited
- → Neuromuscular disruption with ultrashort electrical pulses(2006)11 cited
- → <title>Influence of the pulse duration on laser-induced mechanical effects</title>(1994)11 cited
- → A Section of a Serial Summator of High-Power Microsecond-Duration Pulses(2018)1 cited
- Electron optic system for forming 100 MW beam with high current density and microsecond pulse duration for X-band magnicon(1990)