A functionally based model for hydrolysis of cellulose by fungal cellulase

Citations Over TimeTop 10% of 2006 papers

Abstract

A new functionally based kinetic model for enzymatic hydrolysis of pure cellulose by the Trichoderma cellulase system is presented. The model represents the actions of cellobiohydrolases I, cellobiohydrolase II, and endoglucanase I; and incorporates two measurable and physically interpretable substrate parameters: the degree of polymerization (DP) and the fraction of beta-glucosidic bonds accessible to cellulase, F(a) (Zhang and Lynd, 2004). Initial enzyme-limited reaction rates simulated by the model are consistent with several important behaviors reported in the literature, including the effects of substrate characteristics on exoglucanase and endoglucanase activities; the degree of endo/exoglucanase synergy; the endoglucanase partition coefficient on hydrolysis rates; and enzyme loading on relative reaction rates for different substrates. This is the first cellulase kinetic model involving a single set of kinetic parameters that is successfully applied to a variety of cellulosic substrates, and the first that describes more than one behavior associated with enzymatic hydrolysis. The model has potential utility for data accommodation and design of industrial processes, structuring, testing, and extending understanding of cellulase enzyme systems when experimental date are available, and providing guidance for functional design of cellulase systems at a molecular scale. Opportunities to further refine cellulase kinetic models are discussed, including parameters that would benefit from further study.

Related Papers

• → Cellulases and their interaction with cellulose(1994)169 cited
• → Cellulase in Degradation of Lignocellulosic Wastes(2020)3 cited
• → Real‐time detection of the morphological change in cellulose by a nanomechanical sensor(2010)4 cited
• Progress in the Studies of Cellulose Degradation by Cellulase(2007)
• Technological study on cellulose degradation with cellulase by cross experiments(2002)