A novel structured kinetic modeling approach for the analysis of plasmid instability in recombinant bacterial cultures
Citations Over TimeTop 10% of 1989 papers
Abstract
The instantaneous specific growth rate of a recombinant bacterial culture is directly calculated using a simple structured kinetic modeling approach. Foreign plasmid replication and foreign protein expression represent metabolic burdens to the host cell. The individual effects of these plasmid-mediated activities on the growth rate of plasmid-bearing cells are estimated separately. The dynamic and steady state simulations of the model equations show remarkable agreement with widely observed experimental trends in plasmid copy number and foreign protein content. The model provides an important tool for understanding and controlling plasmid instability in recombinant bacterial fermentations. The modeling framework employed here is suitable for studying the metabolism and growth of a variety of microbial cultures.
Related Papers
- → Conjugation efficiency depends on intra and intercellular interactions between distinct plasmids: Plasmids promote the immigration of other plasmids but repress co-colonizing plasmids(2017)89 cited
- → Successful spread of mcr-1-bearing IncX4 plasmids is associated with variant in replication protein of IncX4 plasmids(2024)7 cited
- [Expression of recombinant human ZP3 protein using the baculovirus expression system].(2014)
- Genetic and molecular characterization of pR351 plasmid from enteropathogenic Escherichia coli strain.(1989)
- → Successful spread of mcr-1 -bearing IncX4 plasmids is associated with variant in replication protein of IncX4 plasmids(2023)