Studies on the optimization of growth and indole alkaloid production by hairy root cultures of Catharanthus roseus

Citations Over TimeTop 18% of 1991 papers

Abstract

Catharanthus roseus hairy root cultures, genetically transformed with Agrobacterium rhizogenes, produce a wide variety of indole alkaloids. The effect of sucrose, phosphate, nitrate, and ammonia concentrations on growth and indole alkaloid production of C. roseus hairy root cultures were studied by using statistical experimental designs and linear regression analysis. Contradictory effects of these nutrients on growth and indole alkaloid production were found. The maximal growth was obtained by having 77. 8 mg NaH(2)PO(4) . H(2)O/L and 1. 311 g KNO(3)/L in the medium, whereas the specific production of alkaloids was highest at the lowest levels of all the nutrients studied. The maximal dry weight was obtained with high values of sucrose and ammonia, but clear optimum concentrations could not be found. When having enough nutrients to support reasonable growth, it appeared difficult to affect the specific alkaloid production rates considerably. The growth (dry wt.) with the optimized nutrient concentrations in the medium was more than 50% better than in the control medium with about the same alkaloid production.

Related Papers

• → Manipulating indole alkaloid production by Catharanthus roseus cell cultures in bioreactors: from biochemical processing to metabolic engineering(2007)130 cited
• [Monomeric indole alkaloids from the aerial parts of Catharanthus roseus].(2010)
• → Indole alkaloid production by hairy root cultures of Catharanthus roseus(1989)79 cited
• → Biosynthesis of the Indole Alkaloids. Cell‐free Systems from Catharanthus roseus Plants(1982)33 cited
• → Metabolism of vindoline, catharanthine HCl, and vincaleukoblastine sulfate by suspension cultures of Catharanthus roseus(1977)10 cited