Nitrite inhibition of denitrification by Pseudomonas fluorescens

Citations Over TimeTop 10% of 1995 papers

Abstract

Using a pure culture of Pseudomonas fluorescens as a model system nitrite inhibition of denitrification was studies. A mineral media with acetate and nitrate as sole electron donor and acceptor, respectively, was used. Results obtained in continuous stirred-tank reactors (CSTR) operated at pH values between 6.6 and 7.8 showed that growth inhibition depended only on the nitrite undissociated fraction concentration (nitrous acid). A mathematical model to describe this dependence is put forward. The maximum nitrous acid concentration compatible with cell growth and denitrification activity was found to be 66 mug N/L. Denitrification activity was partially associated with growth, as described by the Luedeking-Piret equation. However, when the freshly inoculated reactor was operated discontinuosly, nitrite accumulation caused growth uncoupling from denitrification activity. The authors suggest that these results can be interpreted considering that (a) nitrous acid acts as a proton uncoupler; and (b) cultures continuoulsy exposed to nitrous acid prevent the uncoupling effect but not the growth inhibition. Examination of the growth dependence on nitrite concentration at pH 7.0 showed that adapted cultures (grown on CSTR) are less sensitive to nitrous acid inhibition than the ones cultivated in batch. (c) 1995 John Wiley & Sons, Inc.

Related Papers

• → ppGpp accumulation in Pseudomonas aeruginosa and Pseudomonas fluorescens subjected to nutrient limitation and biocide exposure(1999)14 cited
• → In vivo construction of a hybrid pathway for metabolism of 4-nitrotoluene in Pseudomonas fluorescens(1997)27 cited
• → A species of Pseudomonas, a most useful bacterium for cryobiological studies(1971)9 cited
• → Properties of Fluorescent Pigment-ProducingPseudomonasStrains Isolated from Soil and Roots of Fruit Trees(1987)4 cited
• → Growth Promotion Activity And Growth Pattern Of Pseudomonas Fluorescens On Different Solid Media(2022)