An HPLC method for the determination of the free cortisol/cortisone ratio in human urine

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Abstract

Abstract The measurement of the urinary free cortisol–cortisone ratio has been reported to be a sensitive indicator of renal 11 β ‐hydroxysteroid dehydrogenase type 2 (11 β ‐HSD 2) activity. This converts biologically active cortisol to inactive cortisone. A decrease in its activity (e.g. through disease or inhibition caused by a therapeutic agent or a foodstuff) may increase cortisol levels and susceptibility towards hypertension. The method presented here uses a simple isocratic tandem column HPLC system. The method has been validated and found to be robust and reproducible. The lower limit of quantification (LLOQ) was found to be 10 ng/mL for both cortisol and cortisone. Samples of urine ( n = 99) from patients, most of whom were on complex combinations of drugs, were analyzed and 92% of samples were found to give successful results with this method (cortisol and cortisone above LLOQ). The ratio ranged from 0.07 to 5.61. No interferences were noted from the drugs that the patients were taking. It was also found that a morning spot urine sample gave comparable results to 24 h collection samples, thus making sample collection much easier. Copyright © 2007 John Wiley & Sons, Ltd.

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