TET1‐Mediated Oxidation of 5‐Formylcytosine (5fC) to 5‐Carboxycytosine (5caC) in RNA
ChemBioChem2016Vol. 18(1), pp. 72–76
Citations Over TimeTop 10% of 2016 papers
Maria Basanta‐Sanchez, Rui Wang, Zhenzhen Liu, Xiaohan Ye, Minyong Li, Xiaodong Shi, Paul F. Agris, Yubin Zhou, Yun Huang, Jia Sheng
Abstract
It was recently revealed that 5-methylcytosine (5mC) in mRNA, similar to its behavior in DNA, can be oxidized to produce 5-hydroxymethylcytosine (5hmC) and 5-formylcytosine (5fC), implying the potential regulatory roles of this post-transcriptional RNA modification. In this study, we demonstrate the in vitro oxidation of 5fC to 5-carboxycytidine (5caC) by the catalytic domain of mammalian ten-eleven translocation enzyme (TET1) in different RNA contexts. We observed that this oxidation process has very low sequence dependence and can take place in single-stranded, double-stranded, or hairpin forms of RNA sequences, although the overall conversion yields are low.
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