Isolation and characterization of human mesenchymal stem cells derived from synovial fluid by magnetic‐activated cell sorting (MACS)

Citations Over TimeTop 11% of 2017 papers

Abstract

Mesenchymal stem cells (MSCs) are the primary source of cells used for cell-based therapy in tissue engineering. MSCs are found in synovial fluid, a source that could be conveniently used for cartilage tissue engineering. However, the purification and characterization of SF-MSCs has been poorly documented in the literature. Here, we outline an easy-to-perform approach for the isolation and culture of MSCs derived from human synovial fluid (hSF-MSCs). We have successfully purified hSF-MSCs using magnetic-activated cell sorting (MACS) using the MSC surface marker, CD90. Purified SF-MSCs demonstrate significant renewal capacity following several passages in culture. Furthermore, we demonstrated that MACS-sorted CD90+ cells could differentiated into osteoblasts, adipocytes, and chondrocytes in vitro. In addition, we show that these cells can generate cartilage tissue in micromass culture as well. This study demonstrates that MACS is a useful tool that can be used for the purification of hSF-MSCs from synovial fluid. The proliferation properties and ability to differentiate into chondrocytes make these hSF-MSCs a promising source of stem cells for applications in cartilage repair.

Related Papers

• → CD90 Expression Controls Migration and Predicts Dasatinib Response in Glioblastoma(2017)50 cited
• → Association of CD90 Expression by CD14+ Dendritic‐Shaped Cells in Rheumatoid Arthritis Synovial Tissue With Chronic Inflammation(2022)7 cited
• → Heterogeneity of The CD90+ Population in Different Stages of Hepatocarcinogenesis(2014)12 cited
• The relationship between tumor stem cells marker CD90 and resistance to cisplatin in human lung adenocarcinoma A549 cells(2013)