Tandem Affinity Purification and Mass Spectrometry (TAP‐MS) for the Analysis of Protein Complexes
Current Protocols in Protein Science2019Vol. 96(1), pp. e84–e84
Citations Over TimeTop 20% of 2019 papers
Abstract
Affinity purification followed by mass spectrometry has become the technique of choice to identify binding partners in biochemical complexes isolated from a physiologic cellular context. In this report we detail our protocol for tandem affinity purification (TAP) primarily based on the use of the FLAG and HA peptide epitopes, with a particular emphasis on factors affecting yield and specificity, as well as steps to implement an automated version of the TAP procedure. © 2019 by John Wiley & Sons, Inc.
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