Immunocytochemical staining of p16ink4a protein as an adjunct test in equivocal liquid‐based cytology

Citations Over TimeTop 24% of 2008 papers

Abstract

For cervical cancer screening, HPV-DNA test is expensive and is not easily available in all clinical situations. Thus, we investigated the role of p16(ink4a) immunostaining as another adjunct test to diagnose cervical neoplasia in equivocal liquid based cytology. Eighty-seven patients were randomly selected for this study (3 patients with normal, 84 patients with abnormal including 24 ASCUS, 30 LSIL, and 30 HSIL). We performed p16(ink4a) immunostaining on ThinPrep slide and on each case from the corresponding cervical biopsy tissues. High-risk HPV-DNA testing was also performed on all the subjects. We found that the immunoreactivity of p16(ink4a) is strongly correlated with the grade of cytologic and histologic diagnoses as well as with Hybrid Capture 2. In comparing the p16(ink4a) immunostaining with the Hybrid Capture 2 for accuracy of the diagnosis of CIN II/III or a higher-grade disease in the case of ASCUS/LSIL on ThinPrep, no significant differences were observed. Our data implies that p16(ink4a) immunocytochemical staining in liquid-based cytology specimens might be used as a good adjunct test to predict cervical histology in equivocal ThinPrep tests.

Related Papers

• → Impact of Liquid-Based Gynecologic Cytology on an HIV-Positive Population(2004)10 cited
• → Is the human papillomavirus test in combination with the Papanicolaou test useful for management of patients with diagnoses of atypical squamous cells of undetermined significance/low-grade squamous intraepithelial lesions?(2001)13 cited
• Comparison of RR100, R10 and morphologic guided list criteria in rescreening of 4000 cervical smears - an experience in a tertiary care hospital(2015)
• Analysis on liquid-based cytology test in the screening of cervical cancer(2009)
• Application of High-risk HPV Combined with Liquid-based Cytology Technology in the Diagnosis of Cervical Lesions(2014)