Transcriptomic comparison of invasive bigheaded carps (Hypophthalmichthys nobilis and Hypophthalmichthys molitrix) and their hybrids

Citations Over TimeTop 19% of 2016 papers

Abstract

Bighead carp (Hypophthalmichthys nobilis) and silver carp (Hypophthalmichthys molitrix), collectively called bigheaded carps, are invasive species in the Mississippi River Basin (MRB). Interspecific hybridization between bigheaded carps has been considered rare within their native rivers in China; however, it is prevalent in the MRB. We conducted de novo transcriptome analysis of pure and hybrid bigheaded carps and obtained 40,759 to 51,706 transcripts for pure, F₁ hybrid, and backcross bigheaded carps. The search against protein databases resulted in 20,336-28,133 annotated transcripts (over 50% of the transcriptome) with over 13,000 transcripts mapped to 23 Gene Ontology biological processes and 127 KEGG metabolic pathways. More transcripts were detected in silver carp than in bighead carp; however, comparable numbers of transcripts were annotated. Transcriptomic variation detected between two F₁ hybrids may indicate a potential loss of fitness in hybrids. The neighbor-joining distance tree constructed using over 2,500 one-to-one orthologous sequences suggests transcriptomes could be used to infer the history of introgression and hybridization. Moreover, we detected 24,792 candidate SNPs that can be used to identify different species. The transcriptomes, orthologous sequences, and candidate SNPs obtained in this study should provide further knowledge of interspecific hybridization and introgression.

Related Papers

• → Comparative studies on the feeding selectivity of silver carpHypophthalmichthys molitrixand bighead carpAristichthys nobilis(1994)68 cited
• → Swimming performance of adult bighead carpHypophthalmichthys nobilis(Richardson, 1845) and silver carpH. molitrix(Valenciennes, 1844)(2016)32 cited
• Status of Silver Carp (Hypophthalmichthys molitrix) and Bighead Carp (Hypophthalmichthys nobilis) in Southeastern Oklahoma(2015)
• STUDY ON THE GROWTH OF SILVER CARP AND BIGHEAD CARP IN DIANSHAN(2011)
• CAPTURED STANDARD AND GROWTH CHARACTERISTICS OF SILVER CARP(HYPOPHTHALMICHTHYS MOLITRIX) AND BIGHEAD CARP(ARISTICHTHYS MOBILIS) IN JINSHAHE RESERVOIR(2012)