Application of a redox-proteomics toolbox to Daphnia magna challenged with model pro-oxidants copper and paraquat

Citations Over Time

Abstract

The redox status of cells is involved in the regulation of several cellular stress-response pathways. It is frequently altered by xenobiotics, as well as by environmental stressors. As such, there is an increasing interest in understanding the redox status of proteins in different scenarios. Recent advances in proteomics enable researchers to measure oxidative lesions in a wide range of proteins. This opens the door to the sensitive detection of toxicity targets and helps decipher the molecular impact of pollutants and environmental stressors. The present study applies the measurement of protein carbonyls, the most common oxidative lesion of proteins, to gel-based proteomics in Daphnia magna. Daphnids were exposed to copper and paraquat, 2 well-known pro-oxidants. Catalase activity was decreased by paraquat, whereas global measurement of protein carbonyls and thiols indicated no change with treatment. Despite the absence of observed oxidative stress, 2-dimensional electrophoresis of the daphnid proteins and measurement of their carbonylation status revealed that 32 features were significantly affected by the treatments, showing higher sensitivity than single measurements. Identified proteins affected by copper indicated a decrease in the heat-shock response, whereas paraquat affected glycolysis. The present study demonstrates the applicability of redox-proteomics in daphnids, and indicates that the heat-shock response plays a counterintuitive role in metal resistance in daphnids.

Related Papers

• → Toxicity and bioaccumulation of TiO2 nanoparticle aggregates in Daphnia magna(2009)522 cited
• → Water fleas require microbiota for survival, growth and reproduction(2014)162 cited
• → The acute toxicity of surfactants on fish cells, Daphnia magna and fish—A comparative study(2000)159 cited
• → A comparison of the toxicity of 50 reference chemicals to freshly isolated rainbow trout hepatocytes and Daphnia magna(1994)112 cited
• → The Apparent Thresholds of Toxicity to Daphnia Magna for Chlorides of Various Metals When Added to Lake Erie Water(1950)126 cited