Overexpression of forkhead box J2 can decrease the migration of breast cancer cells
Citations Over TimeTop 10% of 2012 papers
Abstract
The prognosis of breast cancer patients with metastases is generally poor, so it is essential to elucidate related molecules mechanisms. Forkhead Box J2 (FOXJ2) is a member of Forkhead Box transcription factors, many of which have been reported to participate in tumor migration and invasion. In this study, we showed the expression of FOXJ2 was higher in primary breast cancer tissues without lymph nodes metastases than those with, and there was statistical significance between the expression of FXOJ2 and the clinical factors. Hence, we identified a novel function of metastasis, which was not previously known for FOXJ2. Overexpression of FOXJ2 decreased the motility property of highly migrative MDA-MB-231 cells in vitro by wound healing assays and trans-well migration assays, and it was concurrent with the increased expression of epithelial marker E-cadherin and the decreased expression of mesenchymal marker vimentin by Western blot analysis, reverse transcription PCR analysis, and immunofluorescence analysis. Consistent with these observations, the repression of FOXJ2 in weakly metastatic MCF-7 cells remarkably promoted cellular motility. Our study demonstrates that FOXJ2 can inhibit the metastasis of human breast cancer by regulating the EMT key markers E-cadherin and vimentin.
Related Papers
- → Identification of Claudins by Western Blot and Immunofluorescence in Different Cell Lines and Tissues(2011)13 cited
- [Detection of antibodies to HTLV-III: a comparison of various ELISA methods as screening tests and the Western Blot with immunofluorescence as confirmatory procedures].(1987)
- Effects of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside on expression of vimentin in aorta of atherosclerotic rats(2013)
- [Immunofluorescence study of intermediate filament protein vimentin of CP-activated macrophages].(1990)
- Dexamethasone impact on vimentin in human lens epithelial cells(2016)