MicroRNA‐126 regulates EPCs function: Implications for a role of miR‐126 in preeclampsia

Citations Over TimeTop 10% of 2013 papers

Abstract

Preeclampsia is a specific vascular complication in pregnancy, which has a pathophysiology of altered endothelial homeostasis. There is extensive evidence that endothelial progenitor cells (EPCs) dysfunction underlies the endothelial cells loss that occurs during preeclampsia. MicroRNA-126 (miR-126), an angiogenesis-related miRNA, has been shown to have potential angiogenic effects both in cultured endothelial cells in vitro and ischemia-induced angiogenesis in vivo. However, whether miR-126 has therapeutic potential in placental vasculogenesis of preeclampsia remains unclear. In this report, we analyzed the EPCs number and expression of miR-126 in patients with preeclampsia, then investigated the effects of miR-126 on EPCs function and rat placenta by employing up-regulation and down-regulation strategies. We confirmed that miR-126 enhanced EPCs proliferation, differentiation and migration. However, a strong reduction in EPCs function was observed in vitro after miR-126 inhibitor transfection. MiR-126 exerts pro-angiogenic functions by suppressing the synthetize of antiangiogenic factors PIK3R2. Similar to miR-126 overexpression, PIK3R2 downregulation promoted EPCs function. In pregnant rats, we also found that miR-126 increased vascular sprouting, placenta and fetus weights. These findings suggest that miR-126 is essential for angiogenic properties of EPCs in vitro and placental vasculogenesis in vivo, providing basis for an alternative therapeutic approach in patients with preeclampsia.

Related Papers

• → Bone Marrow as a Source of Endothelial Cells for Natural and Iatrogenic Vascular Repair(2001)92 cited
• Angiogenesis and vasculogenesis for therapeutic neovascularization.(2003)
• → Therapeutic Potential of Endothelial Progenitor Cells for Cardiovascular Diseases(2005)28 cited
• → Vasculogenesis and Angiogenesis(2003)10 cited
• → Endothelial progenitor cells and neovasculogenesis(2012)4 cited