Direct assay of Aplysia tissues and cells with laser desorption/ionization mass spectrometry on porous silicon
Citations Over TimeTop 10% of 2001 papers
Abstract
Desorption/ionization on porous silicon (DIOS) is a form of laser desorption mass spectrometry that allows for the direct mass analysis of a variety of analytes without the addition of organic matrix. Protocols are described for the direct analysis of exocrine tissue and single neurons using DIOS-MS. The atrial gland of Aplysia californica was blotted on to porous silicon and analyzed with DIOS-MS in the range m/z 1000-4000. The ability to culture invertebrate neurons directly on porous silicon is also presented. Isolated bag cells regenerated neuronal processes in culture on porous silicon. DIOS-MS allowed the direct detection of the peptides contained in individual cultured neurons indicating that with appropriate protocols, DIOS can be used with biological samples with considerable thickness.
Related Papers
- → The cellular basis of behavior in Aplysia(1971)101 cited
- → Localization and Functional Characterization of a Novel Adipokinetic Hormone in the Mollusk, Aplysia californica(2014)32 cited
- → NdWFamide: a novel excitatory peptide involved in cardiovascular regulation of Aplysia☆(2001)22 cited
- → METABOLISM OF PUTATIVE TRANSMITTERS IN INDIVIDUAL NEURONS OF APLYSIA CALIFORNICA: AROMATIC AMINO ACID DECARBOXYLASE(1972)34 cited
- → The Peronian Aplysia, Aplysia peroniana (Pl. 706)(1806)