Isolation of fucoxanthin from edible brown algae by microwave‐assisted extraction coupled with high‐speed countercurrent chromatography

Citations Over TimeTop 10% of 2012 papers

Abstract

A rapid and efficient method for the separation and purification of fucoxanthin from edible brown algae by microwave-assisted extraction coupled with high-speed countercurrent chromatography was developed. The algae were first extracted using microwave-assisted extraction, then the dried extract was dissolved and directly introduced into the high-speed countercurrent chromatography system with a two-phase solvent system consisting of hexane-ethyl acetate-ethanol-water (5:5:6:4, v/v/v/v). The isolation was done in less than 75 min, and a total of 0.83 mg, 1.09 mg, and 0.20 mg fucoxanthin were obtained from 25.0 g fresh Laminaria japonica Aresch, 1.5 g dry Undaria pinnatifida (Harv) Sur, and 15.0 g dry Sargassum fusiforme (Harv) Setch, respectively. The purity of fucoxanthin determined by HPLC was over 90% and its structure was further identified by LC-ESI-MS and (1) H-NMR.

Related Papers

• → Counter-current motion in counter-current chromatography(2014)12 cited
• → Angle rotor countercurrent chromatography(1975)18 cited
• → Solubility Behavior of Phenolic Compounds in Hexane—ethyl Acetate, Hexane—ethyl Myristate, and Hexane-Ethyl Pivalate Cosolvent Systems(1981)6 cited
• → Review on Scale-up of Coil Column Countercurrent Chromatographs(2005)2 cited
• Main Factors on the Separative Effect of pH-zone-refining Countercurrent Chromatography(2005)