0 citations0 references

Collision energies: Optimization strategies for bottom‐up proteomics

Mass Spectrometry Reviews2021Vol. 42(4), pp. 1261–1299

Citations Over TimeTop 10% of 2021 papers

Ágnes Révész, Helga Hevér, Arnold Steckel, Gitta Schlosser, Dániel Szabó, Károly Vékey, László Drahos

Abstract

Mass-spectrometry coupled to liquid chromatography is an indispensable tool in the field of proteomics. In the last decades, more and more complex and diverse biochemical and biomedical questions have arisen. Problems to be solved involve protein identification, quantitative analysis, screening of low abundance modifications, handling matrix effect, and concentrations differing by orders of magnitude. This led the development of more tailored protocols and problem centered proteomics workflows, including advanced choice of experimental parameters. In the most widespread bottom-up approach, the choice of collision energy in tandem mass spectrometric experiments has outstanding role. This review presents the collision energy optimization strategies in the field of proteomics which can help fully exploit the potential of MS based proteomics techniques. A systematic collection of use case studies is then presented to serve as a starting point for related further scientific work. Finally, this article discusses the issue of comparing results from different studies or obtained on different instruments, and it gives some hints on methodology transfer between laboratories based on measurement of reference species.

Citations Over TimeTop 10% of 2021 papers

Abstract

Related Papers