Use of capillary blood to diagnose hereditary spherocytosis
Pediatric Blood & Cancer2012Vol. 59(7), pp. 1299–1301
Citations Over TimeTop 23% of 2012 papers
Renée Crisp, Liliana Solari, Daniel Gammella, Gabriel Schvartzman, María Cristina Rapetti, Hugo Donato
Abstract
We studied 31 children with hemolytic anemia, or with positive family history for hereditary spherocytosis (HS), to assess the reliability of capillary blood samples for the diagnosis. HS was diagnosed in 20 patients. Cryohemolysis (CH) was positive in 94% and eosin-5'-maleimide flow cytometry in 90% of them, whereas flow cytometric osmotic fragility was positive in 94%. Capillary blood sampling showed to be useful for the diagnosis. Simultaneous use of these three tests allows confirming diagnosis in 100% of patients. The use of very small blood volumes (300 µl) allows an earlier diagnosis in neonates and small infants.
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