Robust microarray production of freshly expressed proteins in a human milieu
PROTEOMICS - CLINICAL APPLICATIONS2012Vol. 7(5-6), pp. 372–377
Citations Over TimeTop 10% of 2012 papers
Fernanda Festa, Sean M. Rollins, Krishna M. Vattem, Margarita Hathaway, Phillip Lorenz, Eliseo A. Mendoza, Xiaobo Yu, Ji Qiu, Greg Kilmer, Penny Jensen, Brian Webb, Ed T. Ryan, Joshua LaBaer
Abstract
HCIVT is a robust in vitro transcription and translation system that yields high levels of protein produced in a human milieu. It can be used in applications where protein expression in a mammalian system and high yields are needed. The increased immunogenic response of HCIVT-expressed proteins will be critical for biomarker discovery in many diseases, including cancer.
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