Structure‐based protein engineering for α‐amylase inhibitory activity of plant defensin

Citations Over TimeTop 24% of 2007 papers

Abstract

The structure of a novel plant defensin isolated from the seeds of the mung bean, Vigna radiate, has been determined by (1)H nuclear magnetic resonance spectroscopy. The three-dimensional structure of VrD2, the V. radiate plant defensin 2 protein, comprises an alpha-helix and one triple-stranded anti-parallel beta-sheet stabilized by four disulfide bonds. This protein exhibits neither insecticidal activity nor alpha-amylase inhibitory activity in spite of showing a similar global fold to that of VrD1, an insecticidal plant defensin that has been suggested to function by inhibiting insect alpha-amylase. Our previous study proposed that loop L3 of plant defensins is important for this inhibition. Structural analyses and surface charge comparisons of VrD1 and VrD2 revealed that the charged residues of L3 correlate with the observed difference in inhibitory activities of these proteins. A VrD2 chimera that was produced by transferring the proposed functional loop of VrD1 onto the structurally equivalent loop of VrD2 supported this hypothesis. The VrD2 chimera, which differs by only five residues compared with VrD2, showed obvious activity against Tenebrio molitor alpha-amylase. These results clarify the mode of alpha-amylase inhibition of plant defensins and also represent a possible approach for engineering novel alpha-amylase inhibitors. Plant defensins are important constituents of the innate immune system of plants, and thus the application of protein engineering to this protein family may provide an efficient method for protecting against crop losses.

Related Papers

• Effect of virus diseases on growth components and yield of mung bean (Vigna radiata) and urd bean (Vigna mungo).(1980)
• → EFFECTS OF COKE ON GERMINATION AND GROWTH OFVIGNA RADIATA (L.) R. WILCZEK, VIGNA MUNGO (L.) HEPPERAND VIGNA UNGUICULATA (L.) WALP.(2021)1 cited
• Interspecific hybridization between mung bean vigna radiata and adzuki bean vigna angularis(1978)
• → Comparison of seed proteins in some representatives of the genusVigna(1985)1 cited
• A comparative study of the biochemical compounds found in three species of Vigna (Vigna angularis L., Vigna mungo L. and Vigna radiata L.) under salt stress conditions(2016)