IgY‐binding peptide screened from a random peptide library as a ligand for IgY purification
Citations Over TimeTop 20% of 2017 papers
Abstract
Chicken egg yolk immunoglobulin (IgY) is a functional substitute for mammalian IgG for antigen detection. Traditional IgY purification methods involve multi-step procedures resulting in low purity and recovery of IgY. In this study, we developed a simple IgY purification system using IgY-specific peptides identified by T7 phage display technology. From disulfide-constrained random peptide libraries constructed on a T7 phage, we identified three specific binding clones (Y4-4, Y5-14, and Y5-55) through repeated biopanning. The synthetic peptides showed high binding specificity to IgY-Fc and moderate affinity for IgY-Fc (Kd : Y4-4 = 7.3 ± 0.2 μM and Y5-55 = 4.4 ± 0.1 μM) by surface plasmon resonance analysis. To evaluate the ability to purify IgY, we performed immunoprecipitation and affinity high-performance liquid chromatography using IgY-binding peptides; the result indicated that these peptides can be used as affinity ligands for IgY purification. We then used a peptide-conjugated column to purify IgY from egg yolks pre-treated using an optimized delipidation technique. Here, we report the construction of a cost-effective, one-step IgY purification system, with high purity and recovery. © 2017 The Authors. Journal of Peptide Science published by European Peptide Society and John Wiley & Sons Ltd.
Related Papers
- → Phage display: Concept, innovations, applications and future(2010)480 cited
- → A polystyrene binding target-unrelated peptide isolated in the screening of phage display library(2016)29 cited
- → Biased selection of propagation-related TUPs from phage display peptide libraries(2017)28 cited
- Potential GM-CSF Antagonists Selected from a Peptide Phage Display Library.(1999)
- → Identification of an Indiscriminate Peptide by Phage Display Technology(2020)