Comparing ion‐pairing reagents and sample dissolution solvents for ion‐pairing reversed‐phase liquid chromatography/electrospray ionization mass spectrometry analysis of oligonucleotides
Rapid Communications in Mass Spectrometry2013Vol. 28(4), pp. 339–350
Citations Over TimeTop 19% of 2013 papers
Abstract
The impact of dissolution solvent on the MS signal of oligonucleotides depends on the type of ion-pairing reagent. Buffer combining 15 mM hexylamine and 50 mM hexafluoro-2-propanol produced the highest overall performance for oligonucleotides (10mer to 40mer) with respect to chromatographic resolution and mass detection.
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