Large scale production and semi-purification of kedarcidin in a 1000-L fermentor
Journal of Industrial Microbiology & Biotechnology1994Vol. 13(6), pp. 356–360
Kin S. Lam, DONALD R. GUSTAVSON, Jacqueline M. Veitch, Salvatore Forenza, John T. Ross, David C. Miller, John Roach, William B. Lebherz, Karyol Poole
Abstract
Actinomycete strain ATCC 53650 was grown in a 1000-L fermentor containing 680 L of medium and the production of kedarcidin was monitored by HPLC. The titers of kedarcidin in the fermentor cultures were 0.49-0.53 mg ml-1. A quick and efficient purification method involving the use of anion exchange resin DE23 (batch adsorption-desorption) and an ultrafiltration system yielded high recovery (65% yield) of kedarcidin from the fermentor culture. Over 200 grams of lyophilized kedarcidin of 70% purity was recovered from each of two 1000-L fermentor cultures using this process.
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