Improved processes for the production and isolation of dynemicin A and large-scale fermentation in a 10000-liter fermentor
Journal of Industrial Microbiology & Biotechnology1992Vol. 11(1), pp. 7–12
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Kin S. Lam, Jeffrey A. Titus, Thomas T. Dabrah, D. Kimball, Jacqueline M. Veitch, DONALD R. GUSTAVSON, Bruce Jon Compton, James A. Matson, Salvatore Forenza, John T. Ross, David C. Miller, John Roach, John A. Beutler
Abstract
Supplementing the culture of Micromonospora chersina sp. nov. No. M956-1 with NaI (0.5 mg/l) enhanced the production of dynemicin A by 35-fold in shake flask culture. Homogeneous dynemicin A was obtained from the whole broth extract by Dicalite chromatography, Sephadex LH-20 chromatography and vacuum liquid chromatography. Gram quantities of dynemicin A were obtained from the fermentation of M. chersina sp. nov. No. M956-1 in a 10,000-liter fermentor.
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