High performance liquid chromatographic separation of monoacylglycerol enantiomers on a chiral stationary phase
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Abstract
Abstract High performance liquid chromatographic separation of monoacylglycerol enantiomers as di‐3,5‐dinitrophenylurethane derivatives was carried out on a chiral stationary phase, N‐( S )‐2‐(4‐chlorophenyl)isovaleroyl‐D‐phenylglycine chemically bonded to v ‐aminopropyl silanized silica. Complete separation of the urethane derivatives of racemic monoacylglycerols with saturated acyl groups of C 12 −C 18 was achieved using a stainless steel column (25 cm long) packed with the 5μ particles, an isocratic elution at ambient temperature with a mixture of hexane/ethylene dichloride/ethanol as a mobile phase, and a UV detector. The sn ‐1 enantiomers were eluted ahead of the corresponding sn ‐3 enantiomers. Complete separation of the sn ‐2 isomers from the corresponding enantiomers and partial separation of the enantiomer homologues differing by two acyl carbons also were observed.
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