Effect of 4,7,10,13,16,19‐docosahexaenoic acid on triglyceride accumulation and secretion in rat hepatocytes in culture

Citations Over TimeTop 25% of 1991 papers

Abstract

Abstract We have investigated the effect of oleic (18∶1) and 4,7,10,13,16,19‐docosahexaenoic (22∶6ω3) acids on triglyceride (TG) accumulation, secretion and reuptake in rat hepatocytes in culture. We also calculated the percentage of total TG, TG‐esterified 18∶1 and TG‐esterified 22∶6ω3 that were secreted relative to the total accumulation (intra+extracellular TG). Both fatty acids were incorporated mainly in the intracellular TG fraction. Treatment with 18∶1 but not with 22∶6ω3 increased the quantity of TG secreted into the culture medium relative to controls. Treatment with 22∶6ω3 caused a greater accumulation of intracellular TG than 18∶1. This arises in part from the preferential retention of 22∶6ω3‐enriched TG by the hepatocytes. At 24 hr, there was no longer any difference in the net secretion of TG by 18∶1 and 22∶6ω3‐treated cells, which may be a consequence of the reuptake of TG‐esterified 18∶1. There was no reuptake of TG‐esterified 22∶6ω3. We conclude that inhibition of hepatocyte TG synthesis is not obligatory for 22∶6ω3‐induced diminution of TG secretion.

Related Papers

• → The lipids of the aging human brain(1967)31 cited
• → Effect of dietary α‐linolenate/linoleate balance on endotoxin‐induced hepatitis in mice(1991)12 cited
• → The effect of dietary n−6 and n−3 polyunsaturated fatty acids on blood pressure and tissue fatty acid composition in spontaneously hypertensive rats(1989)42 cited
• → Erratum to: Hypercholesterolemia downregulates autophagy in the rat heart(2017)2 cited
• → Stereospecific analysis of human plasma triglycerides(1974)6 cited