Continuous synthesis of glycerides by lipase in a microporous membrane bioreactor

Citations Over TimeTop 11% of 1984 papers

Abstract

Abstract A membrane bioreactor was developed for continuous synthesis of glycerides by lipase to overcome the drawbacks associated with the usual operation in an emulsion system. One unit (total area: 726 cm 2 ) of flat, plate‐type dialyzer was used as the membrane bioreactor at 40 C. The glycerol solution, containing bacterial lipase and water, was supplied continuously to 1 side of a sheet of microporous polypropylene membrane (strongly hydrophobic) and the effluent was recycled, while undiluted liquid fatty acid (oleic or linoleic) was fed continuously to the opposite side of the membrane and came in contact with a glycerol‐water‐lipase solution to cause the reaction. The product, glycerides, was obtained at the outlet, in a pure state, with no other phase. Highest conversion (ca. 90%) was obtained when the water content of the glycerol solution was 3–4%. As the accumulation of water produced by the reaction lowered the conversion, molecular sieves in a column that the glycerol solution passed through were used for optimal water content. The reaction could be continued at least for 1 month, yielding a conversion above 70% when 1% CaCl 2 was added in the glycerol solution. The main component of glycerides formed was almost equimolar amounts of mono‐and diglycerides.

Related Papers

• → Lipase-catalyzed reactions of fatty acids with glycerol and acylglycerols(1989)41 cited
• → Enrichment of polyunsaturated fatty acids withGeotrichum candidum lipase(1994)113 cited
• → A novel lipase hydrolysis method to concentrate erucic acid glycerides in Cruciferae oils(1993)18 cited
• → Biocatalyzed acidolysis of soybean oil triacylglycerols to increase oleic acid content(2004)12 cited
• → Bovine Pancreatic Lipase . III. Lipolysis of Oils and Fats and Fatty Acid Specificity(1976)13 cited