Cytochemical and electron-microscopic investigation of the golgi apparatus and golgi-resembling inclusions in the root meristem ofFagopyrum esculentum moench
Abstract
1. The classical cytological methods, cytochemistry and electron microscopy were used to study the cytoplasm ofFagopyrum esculentum Moench. 2. Cytochemical investigation of inclusions resembling the Golgi apparatus was possible after fixation in Ca-formaldehyde and embedding in carbowax. 3. A characteristic of the inclusions was drawn up. The inclusions resemble some pigment substances of animal cells cytochemically (lipopigments, phenolic pigments). 4. Conditions of staining inclusions with basic dyes, Sudan black B and the PAS reaction were studied. 5. A functional investigation revealed a cycle resembling that known in the formation of secretions in animal cells. 6. The Golgi apparatus and inclusions resembling it appear under the electron microscope as two distinct components.
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