Quantitative Map of Proteome Dynamics during Neuronal Differentiation
Citations Over TimeTop 10% of 2017 papers
Abstract
Neuronal differentiation is a multistep process that shapes and re-shapes neurons by progressing through several typical stages, including axon outgrowth, dendritogenesis, and synapse formation. To systematically profile proteome dynamics throughout neuronal differentiation, we took cultured rat hippocampal neurons at different developmental stages and monitored changes in protein abundance using a combination of stable isotope labeling and high-resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS). Almost one third of all 4,500 proteins quantified underwent a more than 2-fold expression change during neuronal differentiation, indicating extensive remodeling of the neuron proteome. To highlight the strength of our resource, we studied the neural-cell-adhesion molecule 1 (NCAM1) and found that it stimulates dendritic arbor development by promoting actin filament growth at the dendritic growth cone. We anticipate that our quantitative map of neuronal proteome dynamics is a rich resource for further analyses of the many identified proteins in various neurodevelopmental processes.
Related Papers
- → Tau is required for neurite outgrowth and growth cone motility of chick sensory neurons(1999)94 cited
- → Collapse of growth cone structure on contact with specific neurites in culture(1987)281 cited
- Neurexin-1α regulates neurite growth of rat hippocampal neurons.(2019)
- → Growth cones: The mechanism of neurite advance(1991)44 cited
- → Neural cell adhesion molecule‐mediated neurite outgrowth is repressed by overexpression of HES‐1(2002)12 cited