Construction and Analysis of Two Genome-Scale Deletion Libraries for Bacillus subtilis

Citations Over TimeTop 1% of 2017 papers

Abstract

A systems-level understanding of Gram-positive bacteria is important from both an environmental and health perspective and is most easily obtained when high-quality, validated genomic resources are available. To this end, we constructed two ordered, barcoded, erythromycin-resistance- and kanamycin-resistance-marked single-gene deletion libraries of the Gram-positive model organism, Bacillus subtilis. The libraries comprise 3,968 and 3,970 genes, respectively, and overlap in all but four genes. Using these libraries, we update the set of essential genes known for this organism, provide a comprehensive compendium of B. subtilis auxotrophic genes, and identify genes required for utilizing specific carbon and nitrogen sources, as well as those required for growth at low temperature. We report the identification of enzymes catalyzing several missing steps in amino acid biosynthesis. Finally, we describe a suite of high-throughput phenotyping methodologies and apply them to provide a genome-wide analysis of competence and sporulation. Altogether, we provide versatile resources for studying gene function and pathway and network architecture in Gram-positive bacteria.

Related Papers

• → ВПЛИВ BACILLUS SUBTILIS НА ПОРОСЯТ НА ВІДЛУЧЕННІ(2022)4 cited
• → СВОЙСТВА ШТАММОВ BACILLUS SUBTILIS КАК ПРОДУЦЕНТОВ АМИЛАЗ ПРИ ПРОИЗВОДСТВЕ САХАРОСОДЕРЖАЩЕЙ КОРМОВОЙ ДОБАВКИ(2020)1 cited
• 산란계에 Bacillus subtilis의 급여가 계란 품질, 혈액 성상 및 분내 암모니아태 질소 함량에 미치는 영향(2005)
• 한외여과 막에 의한 대장균과 Bacillus Subtilis균의 제거(2015)
• → ИСПОЛЬЗОВAНИЕ ПОТЕНЦИAЛA СОЦИAЛЬНЫХ ПAРТНЕРОВ В ПОДГОТОВКЕ БУДУЩИХ ПЕДAГОГОВ(2024)