A single-cell transcriptomic inventory of murine smooth muscle cells

Citations Over TimeTop 10% of 2022 papers

Abstract

Smooth muscle cells (SMCs) execute important physiological functions in numerous vital organ systems, including the vascular, gastrointestinal, respiratory, and urogenital tracts. SMC differ morphologically and functionally at these different anatomical locations, but the molecular underpinnings of the differences remain poorly understood. Here, using deep single-cell RNA sequencing combined with in situ gene and protein expression analysis in four murine organs-heart, aorta, lung, and colon-we identify a molecular basis for high-level differences among vascular, visceral, and airway SMC, as well as more subtle differences between, for example, SMC in elastic and muscular arteries and zonation of elastic artery SMC along the direction of blood flow. Arterial SMC exhibit extensive organotypic heterogeneity, whereas venous SMC are similar across organs. We further identify a specific SMC subtype within the pulmonary vasculature. This comparative SMC cross-organ resource offers insight into SMC subtypes and their specific functions.

Related Papers

• → Predicting tissue-specific gene expression from whole blood transcriptome(2021)87 cited
• → Transcriptome analysis of human ageing in male skin shows mid-life period of variability and central role of NF-κB(2016)61 cited
• → Characterization of Early Developments in the Splenic Leukocyte Transcriptome of NOD Mice(2003)5 cited
• → TRANSCRIPTOMIC AGING IN THE HRS(2019)
• → Studying gene expression profiles in specialized brain regions by microSAGE(2000)