Determination of bioactive peptides using capillary zone electrophoresis/mass spectrometry
Analytical Chemistry1991Vol. 63(2), pp. 109–114
Citations Over TimeTop 1% of 1991 papers
Abstract
Mixtures of bioactive peptides have been analyzed by capillary zone electrophoresis/mass spectrometry (CZE/MS) using an on-line coaxial continuous-flow fast atom bombardment interface. High separation efficiencies (up to 410,000 theoretical plates) were obtained from low femtomole levels of peptides. The analysis of basic peptides was accomplished by using aminopropyl-silylated CZE columns to minimize zone broadening due to adsorption effects. CZE/MS/MS data were acquired from femtomole levels of peptides in electrophoretic real time.
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