Scanning electrochemical microscopy. 23. Retention localization of artificially patterned and tissue-bound enzymes
Analytical Chemistry1993Vol. 65(24), pp. 3598–3604
Citations Over TimeTop 10% of 1993 papers
Abstract
The scanning electrochemical microscope (SECM), operating in the feedback mode, was used to image localized surface reactions of redox enzymes at the micrometer level. Surfaces imaged with the SECM included glucose oxidase immobilized within 8-microns-diameter pores of a filtration membrane and individual whole mitochondria with active NADH cytochrome reductase enzymes in their outer membranes. Factors influencing enzyme image resolution and specificity are discussed.
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