Potentiometric biosensor employing catalytic antibodies as the molecular recognition element

Citations Over TimeTop 10% of 1990 papers

Abstract

Catalytic antibodies are introduced as an important new class of biomolecules for molecular recognition in biosensors in which the binding sites are continually regenerated by the catalytic reaction of the substrate. Consequently, molecular recognition by catalytic antibodies can yield reversible immunoblosensors. In this example, a prototype potentiometric biosensor is described in which a micro-pH electrode is modified with a catalytic antibody that catalyzes the hydrolysis of phenyl acetate, producing hydrogen ions that can be monitored by the electrode. The reversible response is linear with the log of substrate concentration over a range of 20-500 microM with a detection limit of 5 microM under the conditions of this study. Alternative applications of catalytic antibodies in other biosensor configurations are discussed.

Related Papers

• → Water-soluble ZnO–Au nanocomposite-based probe for enhanced protein detection in a SPR biosensor system(2010)52 cited
• → The use of ion-selective electrodes in the determination of drug substances(1989)79 cited
• → The potentiometric and spectrophotometric determination of dissociation constants for same 2-mercapto-5-R-amino-1,3,4-thiadiazole derivatives(1998)13 cited
• → Determination of Dissociation Constants of (5,6-Dioxo-3-sulfoquinoline-7-yloxy)acetic Acid in Aqueous Solution at 25°C by Potentiometric Titration(2020)
• → DETERMINING THE THERMODYNAMIC DISSOCIATION CONSTANTS OF 5-BROMO-6,7-DIHYDROXY-N-METHYL-3-SULFOQUINOLINE IN AQUEOUS SOLUTION AT 25 OC BY POTENTIOMETRIC METHOD(2022)