Application of Avidin−Biotin Technology and Adsorptive Transfer Stripping Square-Wave Voltammetry for Detection of DNA Hybridization and Avidin in Transgenic Avidin Maize
Analytical Chemistry2003Vol. 75(11), pp. 2663–2669
Citations Over TimeTop 10% of 2003 papers
Michal Masařík, René Kizek, Karl J. Kramer, Sabina Billová, Marie Brázdová, Jan Vacek, Michele Bailey, František Jelen, John A. Howard
Abstract
The proteins streptavidin and avidin were electrochemically detected in solution by adsorptive transfer stripping square wave voltammetry (AdTS SWV) at a carbon paste electrode (CPE). AdTS SWV was used to quantify biotinylated oligonucleotides, DNA hybridizations, and avidin in extracts of transgenic avidin maize. The detection limits of denatured and native streptavidin were 6 pM and 120 nM, respectively. The results demonstrated that streptavidin/avidin AdTS SWV is a sensitive and specific method for quantifying DNA and proteins in biological samples such as foods and tissue extracts, including genetically modified crops (avidin maize) and other plants in neighboring fields.
Related Papers
- → Switchavidin: Reversible Biotin–Avidin–Biotin Bridges with High Affinity and Specificity(2014)36 cited
- → A comparison of the binding of biotin and biotinylated macromolecular ligands to an anti-biotin monoclonal antibody and to streptavidin(1993)25 cited
- → Methods to determine biotin-binding capacity of streptavidin-coated magnetic particles(1999)21 cited
- → Cellular Uptake of Fluorescent Labelled Biotin–Streptavidin Microspheres(2008)16 cited
- → Oligonucleotide-Blocked Streptavidin for Biotinylation Analysis(2022)2 cited