Microfluidic Device for Single-Cell Analysis
Analytical Chemistry2003Vol. 75(14), pp. 3581–3586
Citations Over TimeTop 1% of 2003 papers
Aaron R. Wheeler, William Throndset, Rebecca J. Whelan, Andrew M. Leach, Richard N. Zare, Yish Hann Liao, K Farrell, Ian D. Manger, Antoine Daridon
Abstract
We have developed a novel microfluidic device constructed from poly(dimethylsiloxane) using multilayer soft lithography technology for the analysis of single cells. The microfluidic network enables the passive and gentle separation of a single cell from the bulk cell suspension, and integrated valves and pumps enable the precise delivery of nanoliter volumes of reagents to that cell. Various applications are demonstrated, including cell viability assays, ionophore-mediated intracellular Ca2+ flux measurements, and multistep receptor-mediated Ca2+ measurements. These assays, and others, are achieved with significant improvements in reagent consumption, analysis time, and temporal resolution over macroscale alternatives.
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