Monolithic Silica-Based Capillary Reversed-Phase Liquid Chromatography/Electrospray Mass Spectrometry for Plant Metabolomics
Analytical Chemistry2003Vol. 75(23), pp. 6737–6740
Citations Over TimeTop 10% of 2003 papers
Abstract
Application of C18 monolithic silica capillary columns in HPLC coupled to ion trap mass spectrometry detection was studied for probing the metabolome of the model plant Arabidopsis thaliana. It could be shown that the use of a long capillary column is an easy and effective approach to reduce ionization suppression by enhanced chromatographic resolution. Several hundred peaks could be detected using a 90-cm capillary column for LC separation and a noise reduction and automatic peak alignment software, which outperformed manual inspection or commercially available mass spectral deconvolution software.
Related Papers
- → Metabolome analysis based on capillary electrophoresis-mass spectrometry(2014)90 cited
- → Capillary electrophoresis-electrospray ionization-mass spectrometry(1989)94 cited
- → Suppression of Protein Structural Perturbations in Native Electrospray Ionization during the Final Evaporation Stages Revealed by Molecular Dynamics Simulations(2021)11 cited
- → Electro-kinetic assisted electrospray ionization for enhanced complex sample analysis(2016)10 cited
- → Pneumatically assisted electrospray-ion mobility spectrometry for quantitative analysis of intact proteins(2012)7 cited