Design of a Compact Disk-like Microfluidic Platform for Enzyme-Linked Immunosorbent Assay
Citations Over TimeTop 1% of 2004 papers
Abstract
This paper presents an integrated microfluidic device on a compact disk (CD) that performs an enzyme-linked immunosorbent assay (ELISA) for rat IgG from a hybridoma cell culture. Centrifugal and capillary forces were used to control the flow sequence of different solutions involved in the ELISA process. The microfluidic device was fabricated on a plastic CD. Each step of the ELISA process was carried out automatically by controlling the rotation speed of the CD. The work on analysis of rat IgG from hybridoma culture showed that the microchip-based ELISA has the same detection range as the conventional method on the 96-well microtiter plate but has advantages such as less reagent consumption and shorter assay time over the conventional method.
Related Papers
- → Effect of Folin Reagent from Different Sources on Protease Determination(2013)1 cited
- → Fluorinated Organocopper Reagents(2011)1 cited
- → A microtiter plate assay for the detection of inhibitors of the Na +, K + -ATpase(1994)1 cited
- STUDIES ON ANALYTICAL FUNCTIONAL GROUPS OF ORGANIC REAGENTS USED IN INORGANIC ANALYSIS——II.ON REAGENTS FOR ANTIMONOUS IONS(1956)
- Performance evaluation of stability of the total bile acid reagent with improved reagent bottles(2011)