Strategy for Profiling and Structure Elucidation of Mucin-Type Oligosaccharides by Mass Spectrometry
Citations Over TimeTop 17% of 2004 papers
Abstract
A strategy combining accurate mass determination, tandem mass spectrometry, structure homology, and exoglycosidases is described that allows the structural characterization of mucin-type O-linked oligosaccharides. The method is used to profile with quantitation the O-linked oligosaccharide (both neutral and anionic) components of the only diploid Xenopus frog, Xenopus tropicalis. Collision-induced dissociation was used to determine connectivity, to identify previously characterized oligosaccharides, and to determine the presence of structural motifs in unknown oligosaccharides. Exoglycosidase digestion was used to identify the individual residues along with the linkages. The enzymes were also used to cleave larger oligosaccharides to smaller units that are similar to previously elucidated components. By using CID, isomeric structures were compared to determine whether they were identical. In this way, the exoglycosidases were more effectively used, and their use was minimized. A total of 35 oligosaccharides including neutral, sialylated, and sulfated were characterized in this way. The relative abundances of all components were also determined based on HPLC.
Related Papers
- → Carbohydrate moieties of procine 32 kDa enamelin(1995)51 cited
- → Structure of the major oligosaccharide of cobra venom factor(1992)31 cited
- → Developmental changes in Asn-linked neutral oligosaccharides in murine cerebrum(1999)5 cited
- → Evidence for the presence and structure of asparagine-linked oligosaccharide units in the core protein of proteodermatan sulphate(1983)17 cited
- → Enzymatic Release of O- and N-Linked Oligosaccharide Chains(2003)