Characterization and Performance of MALDI on a Triple Quadrupole Mass Spectrometer for Analysis and Quantification of Small Molecules

Citations Over TimeTop 10% of 2005 papers

Abstract

The usefulness of MALDI for small-molecule work has been limited by matrix chemical interference in the mass range of interest, tedious sample preparation, and various crystallization and sample deposition issues. We report instrument characterization and small-molecule quantification performance data from a high repetition rate laser MALDI ion source coupled to a triple quadrupole mass spectrometer. The high repetition rate laser improves sensitivity and precision and allows a proportional increase in sample throughput. Tandem mass spectrometry is used to discriminate the signal from the high chemical background caused by the MALDI matrix. Successful quantification requires use of an internal standard and a means of sample cleanup for typical in vitro sample compositions. This instrument combination and analysis technique is relatively insensitive to sample crystal quality and spot homogeneity. Quantitative performance results are characterized for 53 small-molecule pharmaceutical compounds and compared to those obtained by ESI-MS/MS. Further comparison between MALDI and ESI is examined, and the potential for high-throughput MALDI-MS/MS quantification is demonstrated.

Related Papers

• → An Application of Quadrupole Mass Filter to Charge Spectrometer(1976)4 cited
• CO와 H₂ 혼합기체에 대한 Quadrupole Mass Spectrometer의 감도 특성(1993)
• → A New Design for High Sensitive Analysis of Gaschromatograph-Quadrupole Mass Spectrometer(1975)
• METHOD OF MEASUREMENT IN HIGH PRESSURE BY QUADRUPOLE MASS SPECTROMETER(2007)
• A study of ion-molecule reactions in the gas phase using a triple quadrupole mass spectrometer and a quinqua quadrupole mass spectrometer(1989)