Gold-Enhanced Biomolecular Surface Imaging of Cells and Tissue by SIMS and MALDI Mass Spectrometry
Citations Over TimeTop 1% of 2005 papers
Abstract
Surface metallization by plasma coating enhances desorption/ionization of membrane components such as lipids and sterols in imaging time-of-flight secondary ion mass spectrometry (TOF-SIMS) of tissues and cells. High-resolution images of cholesterol and other membrane components were obtained for neuroblastoma cells and revealed subcellular details (resolving power 1.5 mum). Alternatively, in matrix-enhanced SIMS, 2,5-dihydroxybenzoic acid electrosprayed on neuroblastoma cells allowed intact molecular ion imaging of phosphatidylcholine and sphingomyelin at the cellular level. Gold deposition on top of matrix-coated rat brain tissue sections strongly enhanced image quality and signal intensity in stigmatic matrix-assisted laser desorption/ionization imaging mass spectrometry. High-quality total ion count images were acquired, and the neuropeptide vasopressin was localized in the rat brain tissue section at the hypothalamic area around the third ventricle. Although the mechanism of signal enhancement by gold deposition is under debate, the results we have obtained for cells and tissue sections illustrate the potential of this sample preparation technique for biomolecular surface imaging by mass spectrometry.
Related Papers
- → Laser Desorption-Ionization of Lipid Transfers: Tissue Mass Spectrometry Imaging without MALDI Matrix(2010)85 cited
- → Tissue MALDI Mass Spectrometry Imaging (MALDI MSI) of Peptides(2015)29 cited
- → Analysis of Neuropeptides by MALDI Imaging Mass Spectrometry(2013)12 cited
- → Molecular Histology Analysis of Cryopreserved Tissue Using Peptide/Protein MALDI-TOF Imaging Mass Spectrometry (MALDI-IMS)(2021)1 cited
- → Matrix assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) reveals and localizes different lipids classes involved in atherosclerosis development(2014)