Performance of a Linear Ion Trap-Orbitrap Hybrid for Peptide Analysis

Citations Over TimeTop 1% of 2005 papers

Abstract

Proteomic analysis of digested complex protein mixtures has become a useful strategy to identify proteins involved in biological processes. We have evaluated the use of a new mass spectrometer that combines a linear ion trap and an Orbitrap to create a hybrid tandem mass spectrometer. A digested submandibular/sublingual saliva sample was used for the analysis. We find the instrument is capable of mass resolution in excess of 40,000 and mass measurement accuracies of less than 2 ppm for the analysis of complex peptide mixtures. Such high mass accuracy allowed the elimination of virtually any false positive peptide identifications, suggesting that peptides that do not match the specificity of the protease used in the digestion of the sample should not automatically be considered as false positives. Tandem mass spectra from the linear ion trap and from the Orbitrap have very similar ion abundance ratios. We conclude this instrument will be well suited for shotgun proteomic types of analyses.

Related Papers

• → Top-down Protein Sequencing and MS3 on a Hybrid Linear Quadrupole Ion Trap-Orbitrap Mass Spectrometer(2006)198 cited
• → Unique scanning capabilities of a new hybrid linear ion trap mass spectrometer (Q TRAP) used for high sensitivity proteomics applications(2003)146 cited
• → Automated Identification and Quantification of Protein Phosphorylation Sites by LC/MS on a Hybrid Triple Quadrupole Linear Ion Trap Mass Spectrometer(2005)106 cited
• → Quantitative Comparison of Proteomic Data Quality between a 2D and 3D Quadrupole Ion Trap(2006)53 cited
• Comparison of Acquisition Methods for Linear Quadrupole Ion Trap-Fourier Transform Cyclotron Resonance Mass Spectrometer in Shotgun Proteomics(2010)