IMS−IMS and IMS−IMS−IMS/MS for Separating Peptide and Protein Fragment Ions

Citations Over TimeTop 10% of 2006 papers

Abstract

Multidimensional ion mobility spectrometry (IMS-IMS and IMS-IMS-IMS) techniques have been combined with mass spectrometry (MS) and investigated as a means of generating and separating peptide and protein fragment ions. When fragments are generated inside a drift tube and then dispersed by IMS prior to MS analysis, it is possible to observe many features that are not apparent from MS analysis alone. The approach is demonstrated by examining fragmentation patterns arising from electrospray ion distributions of insulin chain B and ubiquitin. The multidimensional IMS approach makes it possible to select individual components for collisional activation and to disperse fragments based on differences in mobility prior to MS analysis. Such an approach makes it possible to observe many features not apparent by MS analysis alone.

Related Papers

• → Comparison of CCS Values Determined by Traveling Wave Ion Mobility Mass Spectrometry and Drift Tube Ion Mobility Mass Spectrometry(2018)113 cited
• → Analysis of hazardous chemicals by “stand alone” drift tube ion mobility spectrometry: a review(2020)41 cited
• → Drift-Tube Ion Mobility-Mass Spectrometry for Nontargeted ′Omics(2019)14 cited
• → Optimization design of drift tube for ion mobility spectrometer based on simulation of drift electric field(2012)6 cited
• → Transient simulation of moving ion clouds in time-of-flight ion mobility spectrometers operating with DC and AC fields(2015)5 cited