Gene Transfection of Mammalian Cells Using Membrane Sandwich Electroporation

Citations Over TimeTop 10% of 2007 papers

Abstract

To avoid safety issues such as immune response and cytotoxicity associated with viruses and liposomes, physical methods have been widely used for either in vivo or ex vivo gene delivery. They are, however, very invasive and often provide limited efficiency. Using pEGFP and pSEAP plasmids and NIH 3T3 fibroblasts as models, we demonstrate a new electroporation-based gene delivery method, called membrane sandwich electroporation (MSE). The MSE method is able to provide better gene confinement near the cell surface to facilitate gene transport into the cells and thus shows significant improvement over transgene expression of mammalian cells compared to current electroporation techniques.

Related Papers

• → Effective gene transfer to solid tumors using different nonviral gene delivery techniques: Electroporation, liposomes, and integrin-targeted vector(2002)99 cited
• → A novel cationic liposome formulation for efficient gene delivery via a pulmonary route(2011)43 cited
• → Electrically mediated plasmid DNA delivery to hepatocellular carcinomas in vivo(2000)99 cited
• → Erythrocyte ghost-mediated gene delivery for prolonged and blood-targeted expression(2004)36 cited
• → Cationic Liposome Gene Transfer(2003)3 cited