High-Throughput Lens-Free Blood Analysis on a Chip

Citations Over TimeTop 10% of 2010 papers

Abstract

We present a detailed investigation of the performance of lens-free holographic microscopy toward high-throughput on-chip blood analysis. Using a spatially incoherent source that is emanating from a large aperture, automated counting of red blood cells with minimal sample preparation steps at densities reaching up to approximately 0.4 x 10(6) cells/muL is presented. Using the same lens-free holographic microscopy platform, we also characterize the volume of the red blood cells at the single-cell level through recovery of the optical phase information of each cell. We further demonstrate the measurement of the hemoglobin concentration of whole blood samples as well as automated counting of white blood cells, also yielding spatial resolution at the subcellular level sufficient to differentiate granulocytes, monocytes, and lymphocytes from each other. These results uncover the prospects of lens-free holographic on-chip imaging to provide a useful tool for global health problems, especially by facilitating whole blood analysis in resource-poor environments.

Related Papers

• → Robust phase aberration compensation in digital holographic microscopy by self-extension of holograms(2019)18 cited
• → Spatio-temporal Multiplexing in Digital Holographic Microscopy and Holographic Displays(2016)1 cited
• → Multi-dimensional digital holographic microscopy(2018)1 cited
• → Holographic optical element for digital lensless holographic microscopy's illumination(2022)
• → Numerical simulation of digital lensless holographic microscopy holograms through a modified Angular Spectrum algorithm(2023)